Areas of Research
& Special Interests:

Synaptic Communication

Computational Neuroscience

Glial Cell Physiology

Neurocircuitry

Selected Publications

Educational &
Academic Interests:

Teaching

NSC 5201

Journal Club

On-Off Amacrine
Cell Simulations

Recent Graduate Students

Personal Interests

Manuel Esguerra & Robert F. Miller
CD38 immunoreactivity in Retinal Glial Cells

• Acutely isolated tiger salamander Muller glial cells were stained using an antibody to the ecto enzyme CD38 to determine the cellular localization of this molecule.
  
• An example of the staining pattern observed from such immunohistochemical experiments are shown in the movie below.
  
  • Left: Overlay image of DIC light microscopic view of tissue whose image is combined with the flouresence image of antibody staining showing restriction of CD38 to the Muller cell membrane.
  • Right: To better convey the sense of the surface membrane staining of the CD38 antibody, a three-dimensional image was reconstructed from 24 adjacent confocal optical sections spaced 0.4 microns apart.
    

• To view the movie:

• On Macintosh or Windows platforms, you will need to have a Quicktime plugin installed in your web browser.
  If you need the plugin, you can get it here.
• On the SGI platform you will need the movieplayer plugin
• We have not determined if the movie is viewable from the Solaris or Linux platforms.