| Eric A. Newman, Dept. of Neuroscience, University of Minnesota |
||
| |
||
| Newman and Bartosch
(J.
Neurosci. Methods,
93:169-175, 1999) describe
a superfusion chamber used to maintain rat and mouse eyecups in a healthy,
responsive state. The following links show the mechanical drawings
used to manufacture the chamber.
Please note: Newman and Bartosch (1999) reported that the ERG b-wave remained healthy for approximately one hour at 37 °C. When eyecups are maintained at 29 °C, preparations remain healthy for many hours. In rat and mouse eyecups at 29 °C, the transretinal ERG b-wave decays by only 25 and 31 percent respectively, five hours after isolation. |
||
| |
||
 |
3-D view of complete chamber. (A) Perspective view of chamber. (B) Enlarged, cross sectional view, showing the everted eyecup (c) held between the two joined halves of the chamber. See Newman and Bartosch (1999) for an explanation of the abbreviations and for dimensions of the chamber. (GIF file, 98 KB.) | |
| |
||
 |
Upper chamber. (GIF file, 87 KB.) | |
|
|
||
 |
Lower chamber. (GIF file, 104 KB.) | |