Areas of Research
& Special Interests:
Educational &
Academic Interests:
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Estimating the number of functional synapses
impinging on retinal ganglion cells
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Activity-dependent dyes were applied to superfused retina-eyecup preparations and, after wash out of the dye, the whole-mounted isolated retina was then viewed via confocal microscopy.
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Activity-dependent staining was shown to occur in presynaptic terminals in the retina (see Miller et al 2001, J. Comp. Neurol.)
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Ganglion cells were back labeled prior to administration of the activity-dependent dye and dual wavelength confocal microscope data were collected from the retinal whole-mount preparation.
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We have constructed a movie showing a 3-D rotation of a back-labeled retinal ganglion cell dendrite and the activity-dependent dye stained synaptic terminals in the same volume of the inner plexiform layer.
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To view the movie:
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- For a PDF file of the article itself, click here.
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